Journal: International Journal of Molecular Sciences

Article Title: The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity Through the cAMP/Rac1 Pathway

doi: 10.3390/ijms252011110

Figure Lengend Snippet: IMD activates the cAMP/Rac1 pathway and alleviates renal peritubular capillary injury in CIAKI rats. ( A , B ): The renal lysates of rats were collected and the expression of Rac1, VE-cadherin, VEGFR2, and GAPDH were detected using Western blotting. ( C ) Kidney sections were stained with CD34 immunohistochemical staining (magnification, ×400, scale 30 μm). ( D ) Kidney sections were stained with CD34 and ICAM1 immunofluorescence staining (magnification, ×200, scale 50 μm). ( E ) The average optical density of CD34 positive staining in each group was quantified ( n = 6). ( E ) Quantification of the average optical density of ICAM1 immunofluorescence ( n = 6). The data were analyzed using ANOVA, followed by Tukey’s multiple comparisons test for subgroup analysis. All data are expressed as mean ± SD, * p < 0.05 compared to the control group, # p < 0.05. For comparison between groups, ns: not significant.

Article Snippet: The antibodies used in this study were cd34 (BM4082, Boster, Wuhan, China) and ICAM1 (A00171, Boster, Wuhan, China).

Techniques: Expressing, Western Blot, Staining, Immunohistochemical staining, Immunofluorescence, Control, Comparison